Product name |
Bovine TNF-¥Á(Tumor necrosis factor alpha) ELISA Kit
|
Catalogue No. |
LEB0008
|
Alias |
TNF-¥á ELISA Kit, DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit
|
Detection method |
Sandwich ELISA, Double Antibody
|
Size |
96T
|
Range |
0.156-10ng/ml
|
Sensitivity |
< 0.094ng/ml
|
Standard curve |
|
Storage |
4 ¡É for 6 months
|
Recovery |
Matrices listed below were spiked with certain level of TNF-¥Á and the recovery rates were calculated by comparing the measured value to the expected amount of TNF-¥Á in samples.
Matrix |
Recovery range(%) |
Average(%) |
serum(n=5) |
86-104 |
96 |
EDTA plasma(n=5) |
85-99 |
92 |
heparin plasma(n=5) |
89-100 |
95 | |
Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNF-¥Á and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
88-104% |
89-101% |
87-105% |
92-105% |
EDTA plasma(n=5) |
85-99% |
90-98% |
83-97% |
86-100% |
heparin plasma(n=5) |
82-96% |
88-97% |
81-98% |
81-100% | |
CV(%) |
Intra-Assay: CV<8% Inter-Assay: CV<10%
|
Note |
For Research Use Only
|