Ä«Å×°í¸®
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Chemokines
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CAT.NO
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LGP-20-028
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PRODUCT
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Eotaxin/CCL11, Human
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SIZE
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20ug, 100ug, 500ug
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PRICE
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KRW 285,000, 880,000, 2,569,000
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.
Technical Parameters
Synonyms |
Small-inducible cytokine A11 |
Species |
9 |
Accession |
P51671 |
GeneID |
6356 |
Source |
Escherichia coli. |
Molecular Weight |
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids. |
Quantity |
5µg/20µg/1000µg |
AA Sequence |
GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP |
Purity |
> 97 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2 ¥ìm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
Endotoxin |
Less than 1 EU/¥ìg of rHuEotaxin/CCL11 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Â -20 ¡ÆC. Further dilutions should be made in appropriate buffered solutions. |
Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 ¡ÆC as supplied. - 1 month, 2 to 8 ¡ÆC under sterile conditions after reconstitution. - 3 months, -20 to -70 ¡ÆC under sterile conditions after reconstitution. |
Usage |
This material is offered by Korea Lugen Sci for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
SDS-PAGE |
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Reference |
1. Ponath PD, Qin S, Ringler DJ, et al. 1996. J Clin Invest. 97:604-12. 2. Jose PJ, Griffiths-Johnson DA, Collins PD, et al. 1994. J Exp Med. 179:881-7. 3. Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, et al. 1996. Nat Med. 2:449-56. 4. Kitamura K, Singer WD, Star RA, et al. 1996. J Biol Chem. 271:7412-5. |
Background |
Human CCL11 is belonging to the CC chemokine family. It is encoded by the gene CCL11. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Human CCL11 is approximately 63 % identical at the amino acid level to murine CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor. |
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